(b) LPS stimulation

Cytokine	ADM (µmol/L)
	Control	0.01	0.1	1
IL-6	326 ± 9	338 ± 4	350 ± 17	328 ± 13
IL-10	551 ± 50	551 ± 10	523 ± 30	472 ± 12
IL-12p70	22 ± 5	15 ± 5	10 ± 3	16 ± 3
GM-CSF	31 ± 3	27 ± 3	19 ± 2*	18 ± 2*
IFN-γ	44 ± 15	36 ± 12	N.D.	N.D.
MCP-1	262 ± 15	260 ± 24	161 ± 12*	149 ± 28*
TNF-α	81 ± 3	72 ± 4	37 ± 3*	28 ± 2*

Murine spleen cells were stimulated with 1 μg/mL anti-CD3 for 1 d (a) or 1 μg/mL LPS for 2 d (b). Adrenomedullin (ADM) was added to culture medium at the indicated concentrations. Cytokines were measured using a Bio-Plex mouse cytokine multiplex kit. Measurements of IL-17A and TNF-α were performed by conventional ELISA. Cytokine productions with no stimulus were 9.9 ± 1.6 in IL-2, 1.6 ± 0.2 in IL-6, 1.7 ± 0.2 in IL-10, and below the detection limit for the others. N = 3-4. *P < 0.05 versus control by Dunnett test.