Figure 6. Representative Coomassie brilliant blue-stained 2-DE gel of proteins extracted from tomato leaves grown under ambient and elevated CO₂ conditions for six days.

Proteins were separated in the first dimension on an IPG strip (pH 4–7) and in the second dimension on a 12% polyacrylamide gel (for details, see “Materials and Methods”). The spots numbered on the gel (1–6) exhibited 5–10-fold differences (P < 0.05) between the two treatments. Three gels from biological repeats for each treatment were used.