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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Nat Methods. 2013 Oct 27;10(12):1219–1224. doi: 10.1038/nmeth.2701

Figure 2. Spinach2 is brighter than Spinach due to improved folding.

Figure 2

(a) Normalized brightness values of Spinach and derivatives. The fluorescence signal from 1 μM RNA and 10 μM DFHBI was measured at 25°C and normalized to Spinach fluorescence. Data represent mean and s.e.m. values for three independent replicates.

(b) Results of folding assay. Data represent mean and s.e.m. values for three independent replicates.

(c) Percent folded values of Spinach and derivatives at 25°C and 37°C. Data represent mean and s.e.m. values for three independent replicates.

(d) Normalized brightness values of Spinach and derivatives. The fluorescence signal from 1 μM RNA and 10 μM DFHBI was measured at 25°C and normalized to Spinach fluorescence. Data represent mean and s.e.m. values for three independent replicates.