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Evaluation of Rh123 transport inhibition mediated by RALT. The efflux of the dye MDR1-Pgp substrate Rh123 in CEM-VBL100 and HL60-DNR MDR cells was monitored in drug-free conditions (blue histogram), in the presence of the potent MDR1-Pgp blocker Vrp (2.5 μg/mL) (green histogram), and following incubation with RALT (red histogram) dissolved in DMSO or H2O at the concentrations shown on the right side of the panels.
