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. 2013 Oct 8;14:106. doi: 10.1186/1471-2350-14-106

Figure 5.

Figure 5

F370L and I523T MARS associate with the multisynthetase complex. A: Coomassie staining of wild-type, F370L and I523T MARS-FLAG purified fractions. MARS-FLAG is indicated (arrow). The asterisk shows a degradation product of MARS (~75 kD), which is more pronounced after purification of wild-type MARS-FLAG. However, the ratio of the 100 kD full length MARS and the partially degraded form was not established in the wild-type and MARS mutants. MARS was not immunoprecipitated from cell lysates that expressed I523T MARS-FLAG with a nonsense mutation in the FLAG tag that abolished binding to the anti-FLAG antibody (see Additional file 3: Figure S2). B: Components of the multisynthetase complex are co-purified upon immuno-isolation of wild-type, F370L or I523T MARS. Western blotting shows the presence of similar levels of MARS, KARS and RARS (upper, middle and lower panel, respectively) after immuno-isolation of MARS-FLAG from HEK293 cells.