Three different groups of flies were exposed to heat-shock induction (red histograms) or not (blue histograms), behaviorally-trained, and tested for 24-hour memory. Heat-shock induction of the dCREB2 blocker with a mutation in the NLS (936) (n=12, p<0.0005) and induction of the bZIP domain of dCREB2 (568) (n=10, p<0.005) both impair long-term memory, while heat-shock does not impair memory in wild type (2U) flies (n=14, p=0.67). Data are presented as means, and the error bars are s.e.m. Comparisons were made using a two sample t-test. Significant differences between groups are indicated as *p<0.01 or **p<0.0005.