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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Nat Cell Biol. 2013 Nov 10;15(12):1464–1472. doi: 10.1038/ncb2868

Figure 4.

Figure 4

The role of MCU in basal metabolism. a) Seahorse X-24 analysis of oxygen consumption rate (OCR) in WT and MCU-/- MEFs under basal conditions or following the addition of oligomycin, the uncoupler FCCP or the electron transport inhibitor antimycin A (n=5). b) Representative tracings of the oxygen consumption observed in isolated hepatic mitochondria given glutamate/malate as a substrate (G/M) followed by ADP (State 3). c) Quantification of the respiratory control ratio (RCR; State 3/State 4 respiration) in WT and MCU-/- mitochondria (p=NS; n=3 independent experiments). d) Average total body oxygen consumption in WT and MCU-/- mice during day and night conditions (n=5 WT and n=6 MCU-/- mice; p=NS between genotypes). All pooled data represents mean +/- S.E.M.