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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Nat Cell Biol. 2013 Nov 10;15(12):1464–1472. doi: 10.1038/ncb2868

Figure 7.

Figure 7

MCU expression is necessary for calcium-induced PTP opening but not required for cell death. a) Only WT mitochondria undergo PTP opening after calcium addition (500 μM) as evidence by a rapid drop in absorbance. Shown is one experiment using heart mitochondria that is representative of three similar experiments. b) Average change in absorbance from three independent experiments using isolated cardiac mitochondria in the presence or absence of CsA (0.2 μM) and Ru360 (3.0 μM). c) Cell viability as measured by Annexin V/PI staining in WT and MCU-/- MEFs following a wide array of challenges including hydrogen peroxide exposure (1 mM), tunicamycin (2 μg/ml), doxorubicin (2 μM), C2-ceramide (100 μM) and thapsigargin (1 μM). The time course and magnitude of cell death was not altered by the absence of MCU expression (p=NS, n=3 per genotype). d) Cytosolic cytochrome C levels in WT or MCU-/- MEFs following the addition of hydrogen peroxide. Tubulin is shown as a loading control. e) Caspase-3 activity was measured under basal conditions or 24 hours after treatment with tunicamycin or doxorubicin (p=NS; n=3). All pooled data represents mean +/- S.E.M.