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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Int J Dev Neurosci. 2013 Sep 24;31(8):10.1016/j.ijdevneu.2013.09.005. doi: 10.1016/j.ijdevneu.2013.09.005

Figure 5.

Figure 5

Simultaneous suppression of Crb1, Crb2a, and Crb2b severely affected the polarity of the hindbrain neuroepithelium but not that of the otic vesicle. A. The combined expression profiles of Crb1, Crb2a and Crb2b in the hindbrain at 20-ss was revealed by using a mixture of Crb antibodies for confocal immunohistochemistry. B-D. The suppression of Crb1 (B), Crb2a (C), Crb2b (D) in embryos treated with morpholinos against these Crb proteins was verified with immnohistochemistry using individual antibodies. The polarity of the neuroepithelium is affected in the morphants as evidenced by mislocalization/misalignment of apical marker ZO-1 and actin bundles. By contrast, the apicobasal polarity of the otic vesicle remains unaffected (arrowheads). The lack of Crb1, Crb2a and Crb2b immunostaining demonstrated the effectiveness of morpholino suppression. Scale bar, 50 ! m.