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. 2013 Sep 27;14:659. doi: 10.1186/1471-2164-14-659

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Copyright © 2013 Dalton et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Identification of DNA binding motifs for the A, B and C zinc finger domains of Fru^M. (A) Consensus DNA binding sequences of the zinc finger DNA binding domains (DBD) of Fru^MA, Fru^MB or Fru^MC identified after ten rounds of SELEX. (B) Oligonucleotides containing the consensus sequences identified by SELEX, or sequences of the same nucleotide content but randomized in order, for DBD^A or DBD^C were incubated with GST-Fru^A DBD or GST-Fru^C DBD, or GST alone and assayed by gel mobility shift assay. GST-Fru^A DBD and GST-Fru^C DBD bound to oligonucleotides containing their respective consensus sequence (Lane 2 and 8) have slower mobility than GST (Lane 4 and 10), GST-Fru^A DBD (Lane 3 and 12), or GST-Fru^C DBD (Lane 6 and 9) incubated with oligonucleotides containing random sequences or the consensus sequence from other zinc finger domains or free oligonucleotides (no protein added in Lanes 1, 5, 7,11).