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. 2013 Dec 4;8(12):e81212. doi: 10.1371/journal.pone.0081212

Figure 5. Recombinant antigen vaccines administered with alum adjuvant prime murine spleen cells for cytokine responses.

Figure 5

One week following the last booster, spleen cells of mice (n = 6) immunized with antigen-free PBS and alum adjuvant or from mice immunized with recombinant proteins (IsdB, Hla, HI, IH) were incubated for 5 days with antigen proteins (5 µg/ml). The supernatants were harvested, and the cytokine levels of (A) IFN-γ, (B) IL-5, and (C) IL-17A were determined. The medians and interquartile ranges are shown. Asterisks indicate significant differences between the vaccinated and control mice (* P<0.05, ** P<0.01). (D) Role of cytokines in splenocyte supernatants after 5 days of co-culture with recombinant proteins in the phagocytic killing of staphylococci. PMNs were primed with supernatants for four hours, and S. aureus (1:10 S. aureus to PMNs) was added for an additional ninety minute incubation period. The bacteria were then plated on agar medium to measure the bacterial survival as CFU. The medians and interquartile ranges are shown. Asterisks indicate significant differences between the immune and control supernatants (* P<0.05, ** P<0.01).