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. 2013 Dec 4;8(12):e81404. doi: 10.1371/journal.pone.0081404

Figure 3. Membrane acquisition by highly activated T cells is antigen independent.

Figure 3

Freshly isolated Tg4 T cells and Tg4 T cells stimulated for 3 days with anti-CD3 and anti-CD28 were co-cultured with biotinylated BMDC. (A) Membrane transfer to activated and freshly isolated Tg4 T cells in the presence or absence of 4Tyr or 4Tyr + anti-MHCII. (B) MFI of streptavidin-APC staining in activated Vs naïve T cells after co-incubation with 4Tyr pulsed BMDC error bars are SD. (C) Membrane transfer to Tg4 T cells stimulated for 3 days with anti-CD3 and anti-CD28 or stimulated for 3 days with anti-CD3 and anti-CD28 prior to rest in 10 U/ml IL-2 for 4 hrs, 24 hrs or 48 hrs were co-cultured with biotinylated BMD in the presence or absence of 4Tyr. Data shown is from one experiment representative of 4 similar experiments.