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. 2013 Dec 4;8(12):e81404. doi: 10.1371/journal.pone.0081404

Figure 4. Detection of Ag-reactive T cells by membrane exchange after in vivo priming.

Figure 4

(A–E) Tg4 CD4+ T cells were transferred to B10.PL hosts 1 day prior to immunisation with CFA-only or CFA+Ac1-9 4Tyr (control mice were left unimmunised). 5 days after immunisation DLN cells were removed CD4+ T cells were purified and co-cultured with biotinylated BMDC in the presence or absence of 4Tyr (A). (B) The proportion of Tg4 T cells in the DLN of immunised VS non-immunised mice (shown as percentage of CD4+). C) Membrane acquisition within the whole CD4+ population of cells co-cultured with BMDC in the presence or absence of 4Tyr. Membrane acquisition by host (D) and donor (E) CD4+ cells co-cultured with BMDC in the presence or absence of 4Tyr. Each data point represents cells from an individual mouse. *p<0.05. ***, p<0.001.