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Scheme illustrating the sgRNA structure and mechanism of the target recognition. sgRNA is expressed under the U6 promoter in A. thaliana, N. benthamiana, O. sativa, T. aestivum and S. bicolor, and under the U3 promoter in O. sativa. The transcript initiation nucleotide in the case of U6p and U3p is “G” and “A”, respectively.
