Table 2.
Primers used in this study
| Primer name | Sequence 5′ to 3′ |
|---|---|
| PDS_gRNA1_BsaIf |
TGTGGTCTCAATTGCCGTTAATTTGAGAGTCCAGTTTTAGAGCTAGAAATAGCAAG |
| PDS_gRNA2_BsaIf |
TGTGGTCTCAATTGTCAAGATGTTTGCTTGCAAGTTTTAGAGCTAGAAATAGCAAG |
| gRNA_AGCG_BsaIr |
TGTGGTCTCAAGCGTAATGCCAACTTTGTAC |
| Cas9_1f |
GAGGAAGACAAAATGGACAAGAAGTACTCCATTGGG |
| Cas9_1r |
GAGGAAGACAAAGTCTCTTCTGATTTGCGAGTCATCCA |
| Cas9_2f |
GAGGAAGACAAGACTATCACTCCCTGGAACTTCGAG |
| Cas9_2r |
GAGGAAGACAAATCTTCTTTGAGCTGTTTCACGGTAACT |
| Cas9_3f |
GAGGAAGACAAAGATTATTTCAAAAAGATTGAATG |
| Cas9_3r |
GAGGAAGACAAGAGACTGTCCCCCTGGCCAGAAACTTG |
| Cas9_4f |
GAGGAAGACAATCTCCACGAGCACATCGCTAATCTTGCAGG |
| Cas9_4r |
GAGGAAGACAAGAAACCAGCTTAGACTTCAGAGTAATA |
| Cas9_5f |
GAGGAAGACAATTTCAGATTTCAGAAAGGACTTTCAG |
| Cas9_5r |
GAGGAAGACAAAGATCCTTTGAGCTTTTCATAGTGGCTGG |
| Cas9_6f |
GAGGAAGACAAATCTCCCGAAGATAATGAGCAGAAG |
| Cas9_6-1r |
GAGGAAGACAAAAGCTCACACCTTCCTCTTCTTCTTGG |
| PDS_MlyIF |
GCTTTGCTTGAGAAAAGCTCTC |
| PDSseqr5 |
TTTAAAGGATTAAAGTCCTTTGTCA |
| M13 forward |
GTTGTAAAACGACGGCCAGT |
| M13 reverse | CAGGAAACAGCTATGACC |