FIG. 5.

Effects of HCD and LPS injection on HIF1 activation in WT and iNOS-deficient mice. (A) Western blot for HIF1α protein and mRNA levels of (B) HIF1α (C) PDK1 (D) Glut-1 (E) VEGF in WT or iNOS^−/− mice after chronic HCD and LPS challenge. Values are expressed as mean±SEM (n=6–9 for each group). *p<0.05, **p<0.01 versus WT group. HIF1 ameliorates liver damage caused by LPS challenge in HCD-fed iNOS-deficient mice. Mice were fed with HCD for 6 weeks followed by hydrodynamic injection of control/HIF1mut. Three days later mice were injected with LPS. Liver damage was assessed by plasma (F) ALT (G) AST levels. Values are expressed as mean±SEM (n=3–4 for each group). *p<0.05. NS, not significant.