Table 2. Actual type I error rates (%) of FITR.

T	L	Δta	Model 1 (R = 5)b	Model 2 (R = 2)	Model 3 (R = 20)	Model 4 (R = 1)	Model 5 (R = 10)
10	2	5	4.99	5.02	4.95	5.12	5.00
100	20	5	5.09	4.95	5.06	5.05	5.00
1000	200	5	5.07	4.95	5.01	5.02	5.24
10	5	2	4.98	4.94	5.06	4.95	4.94
100	5	20	4.98	5.17	5.20	4.90	5.02
1000	5	200	4.87	5.02	5.06	4.89	5.02

Values indicate the actual type I error rates obtained by 100,000 simulations under a nominal significance level of 5%. The initial frequencies for all R +1 loci, x_h,0, are assumed to be 0.5. FITR, frequency increment test with reference loci.

^a$\mathrm{\Delta }t=t_i-t_{i-1} \left(i=1,2,\dots ,L\right)$.

^bThe numbers of reference loci, R, are randomly assigned to each demographic model.