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. Author manuscript; available in PMC: 2013 Dec 5.
Published in final edited form as: Cold Spring Harb Protoc. 2012 May 1;2012(5):10.1101/pdb.prot069351 pdb.prot069351. doi: 10.1101/pdb.prot069351

Figure 1.

Figure 1

Purification and characterization of BR-CaM. (Top left) The crystal structure of CaM (Houdusse et al. 1996) showing the location and orientation of the BR entity. The arrow identifies the carboxyphenyl ring of BR that produces the two diastereoisomer peaks appearing in the figure at the top right. Reverse-phase (C18) HPLC of labeled CaM (top right) and tryptic fragments (bottom left/right).