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Schematic drawing showing the setup of a resonant waveguide grating biosensor-enabled total internal reflection fluorescence microscopy. Here, a 488nm laser light is used to illuminate the biosensor within a well of 384well microplate with the aid of a right angle prism and the biosensor diffractive grating. The evanescent wave excited fluorescence is collected using an objective lens, and focused via a tube lens onto a CCD camera.
