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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Sens Actuators B Chem. 2013 Nov;188:10.1016/j.snb.2013.08.012. doi: 10.1016/j.snb.2013.08.012

Figure 2.

Figure 2

Characterization of the engineered HEK-β2AR-GFP cells. (a-c) Representative fluorescence microscopic images of the engineered cells after overnight culture: (a) epifluorescence image; (b) TIRF image before any treatment; (c) TIRF image after treatment with 1μM isoproterenol for 1hr. For (a-c) the image scale bar is 50 μm. (d-f) The DMR dose responses of isoproterenol in cells after overnight culturing to form monolayer: (d) the parental HEK293 cells; (e) the engineered cells; (f) comparison of the dose-dependent responses of isoproterenol in both types of cells, wherein for the parental cells the DMR amplitude at 50min post stimulation was plotted as a function of isoproterenol dose, and for the engineered cells the DMR amplitudes at both 5min and 50min post stimulation were plotted. For (d-f), the data represents mean±s.d. (n =4).