Table 1.
Plasmids and strains used in this study
| Plasmids | Relevant characteristics | Source/Reference |
|---|---|---|
| pGEM-T Easy |
ampRoripUC (Cloning vector) |
Promega |
| pMV261 |
kanR (Mycobacterial shuttle non-integrative vector) |
Stover et al., 1991 [14] |
| pDS1 |
pGEM-T Easy containing glnA1 coding sequence with native promoter |
This work |
| pDS2 |
pMV261 containing glnA1 coding sequence with native promoter |
This work |
| pDS3 |
pGEM-T Easy containing glnA1 coding sequence with P1 promoter |
This work |
| pDS4 |
pMV261 containing glnA1 coding sequence with P1 promoter |
This work |
| pDS5 |
pMV261 containing glnA1 coding sequence with P2 promoter |
This work |
|
Strains |
Relevant characteristics |
Source/Reference |
| DH5α |
supE44 ΔlacU(Φ80lacZΔM15) hsdR17 rec1 endA1 gyrA96 thi-1 relA1 |
Novagen |
|
M. bovis AN5 |
Wild Type |
ATCC |
|
M. smegmatis mc2 |
Wild Type |
ATCC |
| MSFP |
M. smegmatis containing pDS2 |
This work |
| MSP1 |
M. smegmatis containing pDS4 |
This work |
| MSP2 | M. smegmatis containing pDS5 | This work |
Selection marker resistant to Ampicillin (ampR) and Kanamycin (kanR).