Table 2.
Estimation of PLG
|
Strain |
M. bovis (gm) |
M. smeg (gm) |
MSFP (gm) |
MSP1 (gm) |
MSP2 (gm) |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| LN | HN | LN | HN | LN | HN | LN | HN | LN | HN | |
|
Dry cell weight |
2.78±0.3 |
2.85±0.2 |
3.04±0.4 |
3.3±0.19 |
3.876±0.16 |
3.34±0.18 |
2.98±0.24 |
3.008±0.11 |
3.43±0.14 |
3.07±0.25 |
|
Cell wall weight after sonication |
1.08±0.2 |
1.34±0.1 |
1.24±0.15 |
1.43±0.23 |
1.87±0.11 |
1.56±0.12 |
1.32±0.32 |
1.47±0.07 |
1.36±0.11 |
1.57±0.11 |
|
Insoluble cell wall after SDS extraction and acetone wash |
0.870±0.1 |
0.680±0.08 |
0.768±0.08 |
0.567±0.13 |
1.02±0.2 |
0.98±0.14 |
0.69±0.09 |
0.75±0.08 |
0.62±0.07 |
0.73±0.12 |
|
Poly-L-glutamine pelleted after sucrose gradient centrifugation |
0.070±0.03 |
No Pellet |
No Pellet |
No Pellet |
0.087±0.017 |
No Pellet |
0.078±0.011 |
No Pellet |
0.056±0.02 |
No Pellet |
| Poly-L-glutamine purified after percoll run | 0.069±0.02 | No PLG | No PLG | No PLG | 0.075±0.012 | No PLG | 0.056±0.02 | No PLG | 0.034±0.01 | No PLG |
Data are mean ± SD of triplicate sample and are representative of three independent experiments.