Figure 4.

PCR-based analysis of the crtS expression cassette and hygromycin B resistance cassette integration into the genome of X. dendrorhous. PCR analyses of Xd_1H1S (1S, one additional crtS gene copy), Xd_2H2S (2S, two additional crtS gene copies), and parental UCD 67–385 (WT) strains, and the negative control without DNA (-). A scheme representing the primers sets that were used (in arrows and numbers according to Additional file 1: Table S1) and the DNA target are under each gel photograph. The scheme shading is in accordance with Figure 3. M: Molecular marker, lambda DNA digested with HindIII.