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. 2013 Oct 9;13:84. doi: 10.1186/1472-6750-13-84

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Copyright © 2013 Contreras et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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RT-qPCR expression analysis of crtS in the wild-type strain and crtS-overexpressing transformants. The crtS transcript level was normalized to the actin mRNA and was determined from RNA extracted after 50 h of culture in YM medium from the parental wild-type UCD 67–385 (WT), the heterozygous Xd_1H1S (DHS3/dhs3^::hph+crtS) and homozygous Xd_2H2S (dhs3^::hph+crtS/dhs3^::hph+crtS) strains. Values are the mean ± standard error of at least three independent cultures. (*P ≤ 0.05; Student’s t test).