Table 2.

Strains and plasmids used and built in this work

	Genotype or relevant features	Source or reference
Strains:
E. coli:
DH-5α	F- φ80d lacZΔM15Δ (lacZY-argF) U169 deoR recA1 endA1 hsdR17(rk- mk+) phoA supE44l- thi-1 gyrA96 relA1	[37]
X. dendrorhous:
UCD 67-385	ATCC 24230, wild-type. Diploid strain [31].	ATCC
Xd_1H	(DHS3/dhs3::hph). Heterozygote transformant derived from UCD 67–385 containing an allele of the DHS3 locus with a deletion and a hygromycin B resistance cassette.	This work
Xd_2H	(dhs3::hph/dhs3::hph). Homozygote transformant derived from Xd_1H by DRM [30] with a deletion and a hygromycin B resistance cassette in both alleles of the DHS3 locus.	This work
Xd_1H1S	(DHS3/dhs3::hph+crtS). Heterozygote transformant derived from UCD 67–385 with a deletion in one DHS3 allele and bearing the hygromycin B resistance cassette and the crtS gene expression cassette.	This work
Xd_2H2S	(dhs3::hph+crtS/dhs3::hph+crtS). Homozygote transformant derived from Xd_1H1S by DRM with a deletion in both DHS3 alleles and bearing the hygromycin B resistance cassette and the crtS gene expression cassette.	This work
Plasmids:
pBluescript SK- (pBS)	ColE1 ori; AmpR; cloning vector for blue-white selection	Stratagene
pMN-hph	pBS containing a cassette of 1.8 kb bearing the resistance Hygromycin B (hph) gene under EF-1 α promoter and gpd transcription terminator of X. dendrorhous in its EcoRV site.	[30]
pXd_Ex_crtS	pBS containing the cDNA encoding the X. dendrorhous crtS gene in its EcoRV site.	[26]
pBS-PTEF-crtS-Tact	pBS containing a 2,367 bp cassette bearing the cDNA encoding the X. dendrorhous crtS gene under EF-1α promoter and the actin transcription terminator from X. dendrorhous in the EcoRV site.	This work

ATCC: American Type Culture Collection.

DHS3 [GenBank: JN849376.1]: locus downstream of the X. dendrorhous HIS3 gene [GenBank: JN849374.1].