Figure 1.

DCs show only a partial maturation on 30-kDa and 38-kDa Mtb antigen stimulation. DCs were generated from peripheral blood monocytes of healthy donors and at day 5 of culture stimulated with 10 μg of the indicated Mtb antigens or LPS or left untreated in buffer as a control for 24 h. Then cells were stained at the cell surface for the indicated markers (dotted or straight lines). Gray histograms show the markers on immature DC (control). The experiment is representative of 5 independent experiments.