FIG. 4.

Intracellular localization of hGGT1 and hGGT2. HEK293T cells transfected with hGGT1 or hGGT2 cDNA expression constructs and grown on coverslips were fixed and processed for indirect immunofluorescence, using the GGT129 antibody (GGT, green) and an antibody against the ER marker, calnexin (CNX, red). Nuclei were stained with DAPI (blue). Areas of overlapping GGT129 and calnexin immunofluorescence appear yellow in the overlay panels. Exposure times for GGT images differ among the samples due to the lower expression levels of the hGGT2 proteins. hGGT1 is localized to the cell membrane, and hGGT2 proteins co-localize with calnexin in the ER. Scale bar=20 μm and applies to all images shown. ER, endoplasmic reticulum.