Table 1. Relative amounts of CatchER-Gd3+ complex formation with corresponding ratios.
| [CatchER] (µM) | [Gd3+] (µM) | [CatchER-Gd3+], absorbance at 493 nm | [CatchER-Gd3+], fluorescence at 395 nm | [CatchER-Gd3+], fluorescence at 488 nm | [CatchER]/[Gd3+] |
|---|---|---|---|---|---|
| 40 | 10 | 11.1 ± 2.3 | 6.7 ± 4.4 | 6.5 ± 3.7 | 4.0 |
| 35 | 15 | 18.0 ± 1.4 | 17.5 ± 1.2 | 16.4 ± 1.0 | 2.3 |
| 30 | 20 | 23.0 ± 0.3 | 22.8 ± 1.0 | 20.5 ± 0.7 | 1.5 |
| 25 | 25 | 30.8 ± 3.5 | 32.7 ± 0.5 | 20.0 ± 1.7 | 1.0 |
| 20 | 30 | 15.7 ± 2.2 | 23.6 ± 0.0 | 14.4 ± 0.5 | 0.6 |
| 15 | 35 | 10.4 ± 0.3 | 19.5 ± 0.8 | 11.4 ± 0.5 | 0.4 |
| 10 | 40 | 7.2 ± 0.0 | 13.7 ± 0.6 | 7.8 ± 0.3 | 0.2 |