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. 2013 Dec 4;8(12):e81865. doi: 10.1371/journal.pone.0081865

Figure 5. Effect of acute ACTH treatment on BrdU pulse-chase-labelled cell numbers in the adult mouse adrenal cortex.

Figure 5

BrdU and Ki67-labelled cell numbers in control and 4 h ACTH treatment groups (n=6) of female F1 mice, treated 6 weeks after 1-week BrdU infusion. Mean percentage (±SEM) of total cell numbers are shown for each zone and treatment that were (A) BrdU-positive/Ki67-negative, (B) BrdU-negative/Ki67-positive, (C) BrdU-positive/Ki67-positive (dual-labelled) and (D) BrdU-negative/Ki67-negative (unlabelled). Cells were counted in ZG, outer ZF, inner ZF and ZR in dual-BrdU/Ki67-immunostained left adrenal sections, as described in the Materials and Methods section.