Figure 4.

Comparison of cell proliferation in transfected VSMCs following treatment with NAC, H₂O₂ or U0126. a: cell numbers were counted with a hemocytometer; b: cell proliferation was also measured using the EdU assay; the presented data are the mean ± SD of triplicate determinations repeated in four separate experiments. High glucose/NAC = cells incubated with high glucose and NAC for 5 days; normal glucose/H₂O₂ = cells incubated with normal glucose and H₂O₂ for 5 days; High glucose/U0126 = cells incubated with high glucose and U0126 for 5 days; normal glucose/H₂O₂/U0126 = cells incubated with normal glucose, H₂O₂ and U0126 for 5 days. *P <0.05; **P <0.01 versus corresponding values in untransfected VSMCs.