Table 2.

Analysis of intracellular ROS levels by flow cytometry

	2 days (Relative fold)	4 days (Relative fold)	6 days (Relative fold)
High glucose	100.0 ± 5.7**	122.3 ± 5.1**	116.5 ± 4.2**
Normal glucose	21.2 ± 2.0	24.3 ± 2.3	22.4 ± 2.1

Values are mean ± SD from triplicate determinations repeated in 4 separate experiments. They are showed as relative fold of fluorescence intensity measurement (High glucose/2 days =100).

2 days, 4 days and 6 days = VSMCs incubated with high glucose or normal glucose for 2 days, 4 days and 6 days.

*P <0.05; **P <0.01 versus corresponding values in VSMCs under normal glucose conditions.