Figure 6.

Effects of the Proteasome-Specific Inhibitors on Pollen Tube Growth in Vitro.

Pollen tube growth is shown at left, and the mean length of the pollen tubes under various conditions is shown at right.

(A) Pollen was incubated in PGM (control) or in medium containing 3 μL of S-RNase antibody together with the cross-pollinated style extracts (CPSE) or self-pollinated style extracts (SPSE), whose final concentration was 25 mg/mL.

(B) Pollen was incubated in PGM or in PGM with CPSE or SPSE with 50 μM MG115 or MG132. As a control, a recommended concentration of protease inhibitors cocktail (PIC) and 0.1% DMSO (control) also were used.

(C) Effects of the S-RNase antibody on the pollen tube growth in combination with the proteasomal inhibitors. The pollen tube length was measured microscopically at 2 h after incubation.