Table 3.

ABA-Repressed Genes in Guard Cells

				Experiment 1			Experiment 2			Experiment 3
Affy Probea	Description	AGI Numberb	Clusterc	Ratiod	P-Valuee	Fold Changef	Ratio	P-Value	Fold Change	Ratio	P-Value	Fold Change	Seki et al.g	Hoth et al.h
12100_at	MYB family transcription factor	At2g21650	V	−3.8	0.99997	−13.9	−3.3	0.99999	−9.8	−3.4	0.99997	−10.6
12185_at	Unknown protein	At1g54860	V	−1.8	0.95007	−3.5	−2.1	0.99898	−4.3	−1.7	0.99733	−3.25
12196_at	Unknown protein	At2g28410	V	−1.5	1.00000	−2.8	−1.8	1.00000	−3.5	−2	0.99998	−4.0
12284_at	Somatic embryogenesis receptor kinase	At1g34210	V	−0.8	0.96060	−2	−1.4	0.99977	−2.6	−2	0.99980	−4.0		4.7
12338_at	Pectin methylesterase	At3g14310	V	−4.1	0.99994	−17.1	−1.7	0.99977	−3.2	−2.3	0.97409	−4.9
12381_at	No hits		V	−0.6	0.94811	−2	−0.7	0.99733	−2	−1.5	0.99996	−2.8
12409_at	Small nuclear ribonucleoprotein U1A	At2g47580	V	−0.8	1.00000	−2	−0.9	0.99992	−2	−2.4	1.00000	−5.3
12768_at	Unknown protein	At2g15890	V	−0.9	0.99999	−2	−0.7	0.99995	−2	−1.6	0.99973	−3.0
12773_at	Membrane channel protein	At2g28900	V	−0.9	1.00000	−2	−0.6	1.00000	−2	−1.2	0.99122	−2.3
12843_s_at	Unknown protein	At2g16590	V	−1.0	0.99994	−2	−0.6	0.99947	−2	−0.8	0.50000	−2
12846_s_at	No hits		V	−2.1	1.00000	−4.3	−0.8	0.99999	−2.0	−1.5	1.00000	−2.8
12881_s_at	No hits		V	−1.6	0.99995	−3.0	−0.7	1.00000	−2	−2.3	1.00000	−4.9
12893_at	Alcohol dehydrogenase	At5g43940	V	−1.6	0.99989	−3.0	−0.6	0.99964	−2	−1.6	0.99998	−3.0
13080_at	40S ribosomal protein	At2g21580	V	−0.7	0.99776	−2	−0.6	0.99905	−2	−1.8	1.00000	−3.5
13184_s_at	Thioredoxin	At5g42980	V	−2.3	1.00000	−4.9	−0.6	1.00000	−2.0	−0.9	0.86906	−2
13236_at	Aldolase	At4g39980	V	−0.6	0.97627	−2	−1.2	0.99843	−2.3	−2.1	1.00000	−4.3
13254_at	Farnesyl pyrophosphate synthetase2	At4g17190	V	−0.7	0.99999	−2	−0.7	0.99973	−2	−1.8	1.00000	−3.5
13554_at	β-Ketoacyl-CoA synthase	At2g16280	V	−1.1	0.99853	−2.1	−1.6	1.00000	−3.0	−1.8	0.99776	−3.5
13672_at	No hits		V	−0.9	0.99967	−2	−1	0.99993	−2	−1.4	0.99939	−2.6
14090_i_at	Unknown protein	At2g15830	V	−0.6	0.99820	−2	−0.6	1.00000	−2	−0.7	0.76103	−2
14663_s_at	Glycosyl hydrolase family	At4g24040	V	−1.0	0.99978	−2	−0.6	0.99910	−2	−0.6	0.49254	−2
14667_at	TRP synthase	At5g54810	V	−0.7	0.99977	−2	−0.9	0.99967	−2	−2.3	0.99999	−4.9
14717_s_at	CLC-a chloride channel protein	At5g40890	V	−0.7	0.99993	−2	−1.1	1.00000	−2.1	−2.6	1.00000	−6.1
15105_s_at	Gly-rich RNA binding protein	At2g21660	V	−1.1	0.99992	−2.1	−1.3	1.00000	−2.5	0.2	0.00000	−2	0.8
15194_s_at	GASA4	At5g15230	IV	−0.8	1.00000	−2	−0.9	1.00000	−2	−0.8	0.89823	−2
15213_s_at	Orn carbamoyltransferase	At1g75330	V	−0.8	0.99991	−2	−0.9	1.00000	−2	−2	1.00000	−4.0
15453_at	Unknown protein	At4g14020	V	−0.9	0.99999	−2	−0.7	0.99990	−2	−1.9	1.00000	−3.7
15510_r_at	No hits		V	−1.8	0.82023	−3.5	−0.8	0.99989	−2	−2.1	1.00000	−4.3
15548_at	Unknown protein	At1g11820	V	−0.9	0.99971	−2	−0.7	0.99978	−2	−2.3	1.00000	−4.9
15624_s_at	ADPG pyrophosphorylase	At5g48300	IV	−0.6	0.99931	−2	−1.2	1.00000	−2.3	−2.2	1.00000	−4.6
15626_s_at	Vacuolar sorting receptor	At3g52850	V	−0.6	0.98817	−2	−0.8	0.99998	−2	−1.7	0.99999	−3.2
15703_i_at	Putative trypsin inhibitor	At2g43520	V	−2.6	0.99971	−6.1	−0.8	1.00000	−2.0	−1.8	0.99934	−3.5
16014_at	Unknown protein	At1g75750	V	−0.8	1.00000	−2	−0.8	1.00000	−2	−1.9	0.99999	−3.7
16042_s_at	Cytochrome P450i	At5g05690	V	−0.7	0.99910	−2	−1.3	0.99986	−2.5	−0.7	0.54467	−2	1.2
16051_at	Tubulin β-9 chain	At4g20890	V	−1.2	0.99997	−2.3	−0.9	0.99988	−2	−1.6	0.99990	−3.0
16092_s_at	Potassium channel protein KAT1	At5g46240	V	−2.3	0.99981	−4.9	−1.5	0.99999	−2.8	−2.3	0.99999	−4.9
16118_s_at	WD-40 repeat protein	At2g19520	V	−0.7	0.50000	−2	−0.9	0.99905	−2	−2.5	1.00000	−5.7
16145_at	Vacuolar ATP synthase	At1g75630	V	−1.0	1.00000	−2.0	−0.9	0.99996	−2	−2.9	0.99999	−7.5
16416_at	Plant defensin protein	At2g02130	V	−0.8	0.99989	−2	−1.8	1.00000	−3.5	−0.9	0.92765	−2
16430_at	Glutathione S-transferasej	At2g30860	V	−0.8	0.99733	−2	−1.3	1.00000	−2.5	−2	1.00000	−4.0	1.1
16451_at	Ketol-acid reductoisomerase	At3g58610	V	−1.3	0.99420	−2.5	−1.5	1.00000	−2.8	−1.6	0.99999	−3.0
16458_s_at	No hits		V	−0.9	0.99993	−2	−0.7	0.99980	−2	−1.6	0.70618	−3.0
16624_s_at	Transcription factor	At1g08810	V	−0.6	0.84813	−2	−1.4	1.00000	−2.6	−1.9	0.99997	−3.7
16897_i_at	Unknown protein	At5g15350	V	−0.7	0.99713	−2	−1	0.99992	−2	−2.3	0.99994	−4.9
16985_s_at	Cytosolic ribosomal protein	At3g48930	V	−0.7	1.00000	−2	−0.6	0.99999	−2	−1.9	0.99994	−3.7
16998_at	dTDP-glucose 4-6-dehydratase	At3g62830	V	−0.7	0.99962	−2	−0.6	0.99985	−2	−1.8	0.99998	−3.5
17033_at	Unknown protein	At5g39410	V	−1.0	0.99910	−2.0	−1	0.99999	−2.0	−1.4	0.90472	−2.64
17126_at	WD-40 repeat protein	At5g58230	V	−0.9	0.82023	−2	−0.6	0.99992	−2	−2.1	1.00000	−4.3
17187_at	Putative arginase	At4g08870	V	−3.2	0.99853	−9.2	−1.6	0.99763	−3.0	−1.4	0.99479	−2.64
17215_at	Trypsin inhibitor	At2g43550	V	−0.8	0.94609	−2	−1	0.99950	−2	−2.1	0.99991	−4.3
17237_at	Unknown protein	At2g22840	V	−1.7	0.77836	−3.2	−2.3	0.99926	−4.9	−2.6	0.99973	−6.06
17865_at	NADPH oxidoreductase	At1g75280	V	−1.2	0.97295	−2.3	−2.7	0.99939	−6.5	−1.8	0.99984	−3.48
17957_at	Acetone-cyanohydrin lyase	At2g23600	IV	−1.2	0.99910	−2.3	−3.3	1.00000	−9.8	−2.9	1.00000	−7.46
18063_i_at	No hits		V	−1.2	0.99425	−2.3	−1	0.99915	−2.0	−2.7	0.99997	−6.5
18272_at	Unknown protein	At2g40080	V	−1.1	0.94402	−2.1	−3.9	1.00000	−14.9	−2.7	0.99999	−6.5
18315_s_at	β-Glucosidase	At4g27830	V	−0.7	0.99718	−2	−1.1	0.98873	−2.1	−2.3	0.99999	−4.9
18611_at	Putative pyruvate water dikinase	At5g26570	V	−1.1	0.99823	−2.1	−0.7	0.99843	−2	0.4	0.31342	−2
18678_at	Peptidylprolyl isomerase ROC4	At3g62030	V	−1.8	0.99853	−3.5	−0.9	0.99995	−2	−2.5	1.00000	−5.7	0.19
18687_at	Pyruvate dehydrogenase subunit	At1g59900	V	−0.7	0.99973	−2	−0.8	1.00000	−2	−1.8	1.00000	−3.5
18708_at	Ripening-related protein	At5g62350	V	−1.4	0.99998	−2.6	−2.3	1.00000	−4.9	−2.8	1.00000	−7.0	0.66
18882_at	No hits		V	−0.7	0.99996	−2	−0.7	0.99943	−2	−1.8	1.00000	−3.5
18956_at	Ethylene response sensor	At2g40940	V	−0.7	0.99943	−2	−0.6	0.99992	−2	−1.9	1.00000	−3.7
19171_at	Trypsin inhibitork	At2g43510	V	−1.5	0.99967	−2.8	−0.6	0.99885	−2	−2.5	1.00000	−5.7	2.97
20282_at	Protein kinase	At2g28930	V	−1.3	0.99989	−2.5	−1.3	1.00000	−2.5	−3.2	1.00000	−9.2
20537_at	Extensin	At4g13340	V	−1.6	0.99892	−3.0	−0.9	0.99733	−2	−1.2	0.93019	−2.3
20585_at	Glutaredoxin	At2g47880	V	−1.7	0.99994	−3.2	−1.9	0.94811	−3.7	−1.6	0.99950	−3.03
20709_s_at	Putative 3-oxoacyl reductase	At1g24360	V	−0.6	0.99998	−2	−0.6	0.99998	−2	−2.2	1.00000	−4.6

^aDescribes names of probe set on Affymetrix chip.

^bAGI locus numbers.

^cIndicates clusters in Figure 5.

^dSignal Log value which measures the change in expression level for a transcript between two arrays. This change is expressed as the log₂ ratio. A log₂ ratio of 1 is the same as a Fold change of 2.

^eChange P-value, which measures the probability that the expression levels of a probe in two different arrays are the same. Change P-values of 1.00000 correspond to P-value >0.99995.

^fFold change is calculated using the signal log ratio.

^gObtained from Supplemental Table 5 online (column for 5-h ABA treatment; Seki et al., 2002).

^hObtained from Supplemental Table Repressed.xls online (Hoth et al., 2002).

ⁱCytochrome p450 gene expression is repressed by ABA at 1, 2, 10, and 24 h of ABA treatment but shows ABA induction at 5 h of ABA treatment.

^jGST gene expression is slightly induced by ABA at 1, 5, and 10 h of ABA treatment but repressed by ABA at 2 and 24 h of ABA treatment (see Seki et al., 2002, Supplemental Table 5 online).

^kTrypsin inhibitor gene is induced by ABA (see Seki et al., 2002, Supplemental Table 1 online).