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A) Ten micrograms secretome protein samples were separated on SDS-PAGE gels, transferred to PVDF membranes, and probed with anti-FN1 or FAT1 antibodies. B) The de-glycosylation of the same amount of secreted proteins from MHCC97L and HCCLM3 cells was performed with PNGase F cleavage for 12 h. Proteins were separated on SDS-PAGE and analyzed by western blotting.
