Protein Gel Blot Analysis of L. esculentum Rboh Protein in Leaves Exposed to Select Phytohormones.
Plants (28 d) were placed in solutions containing 50 μM ABA, GA, IAA, BA, or the ethylene precursor ACC. After 24 h, proteins were extracted from the second upper leaf and fractionated (100 μg per lane) by denaturating SDS-PAGE and immunoblotted with antiserum against the C-terminal portion of the L. esculentum Rboh (Sagi and Fluhr, 2001).