Figure 6.
ROS and PIN II Production in the Systemic Leaf of Wild-Type and Antisense Plants 24 h after Wounding.
(A) ROS accumulation in control and systemic leaves of wild-type and antisense (M) L. esculentum plants 5 h after wounding. Plants were imbibed with DAB for 3 h. Subsequently, lower leaves were wounded. Five hours later, leaves from unwounded control plants and upper systemic leaves of wounded plants were assayed for DAB staining. Brown precipitates correlate with the presence of H2O2.
(B) Quantitative analysis of DAB staining. Quantitative measurements were performed as described in the Methods. Each point represents the mean of four terminal leaflets derived from four different plants. Bars represent se.
(C) Protein gel blot analysis of PIN II protein accumulation in control and systemic leaves of wild-type and antisense L. esculentum plants 24 h after wounding. Leaves of the same size and position in unwounded plants served as controls. Proteins were extracted and fractionated (100 μg per lane) by denaturating SDS-PAGE and immunoblotted with antiserum against PIN II.