Table 3.
Substrate Specificities of Acyl-CoA Synthetase Isozymes
| Isozyme | Substrate | Synthetase Activitynmol/min/mg Protein | Ratio of Activities |
|---|---|---|---|
| LACS2 | Palmitate | 4.6 | 1.98 |
| 16-hydroxypalmitate | 9.1 | ||
| LACS4 | Palmitate | 9.4 | 0.45 |
| 16-hydroxypalmitate | 4.2 | ||
| LACS8 | Palmitate | 11.2 | 0.44 |
| 16-hydroxypalmitate | 4.9 | ||
| LACS9 | Palmitate | 2.6 | 0.50 |
| 16-hydroxypalmitate | 1.3 |
Recombinant proteins for Arabidopsis LACS isozymes were produced in E. coli and assayed for activity against palmitate (16:0) and 16-hydroxypalmitate as described in Methods.