Ectopic Expression of CycBMut-GFP Interferes with Cytokinesis, whereas Expression of GFP Alone Does Not Affect Mitotic Progression.
(A) and (B) Time-lapse images of living cells expressing the nondegradable cyclin B1;1-GFP fusion protein (A) or the GFP protein alone (B) from a Dex-inducible promoter during progression through anaphase, telophase, and cytokinesis. Fluorescent images shown at top, and the corresponding differential interference contrast images shown at bottom.