Figure 7.

arr Mutants Are Affected in the Cytokinin Primary Response Pathway.

RNA was extracted from 10-d-old light-grown seedlings treated with 10 nM BA in liquid MS with 1% sucrose for the indicated time. The RNA was analyzed by RNA gel blotting. The blots were probed with either an ARR7, SST1, or β-tubulin radiolabeled probe. The signal obtained for each was quantified using a PhosphorImager, and the ARR7 and SST1 signals were normalized to the β-tubulin signal. The experiment was conducted twice with similar results.