Table 1.

Procedures with blood samples

Time of extraction	Source	Blood sample	Tests to perform	Storage requirements
Birth	Umbilical cord	15 ml EDTA	Stat-Pak.	-
			T. Detect,
			ELISA*,	Filter paper 4°C, Plasma -20°C*
			PCR*	10 ml Guanidine 4°C*
	Umbilical cord	5 ml heparinized	Parasitological examination*	Max 6 hours at 4°C
Before hospital discharge*	Maternal, venous in all (+) subjects	15 ml EDTA	Stat-Pak,	-
			T. Detect,
			ELISA,	Filter paper 4°C, Plasma -20°C,
			PCR	10 ml Guanidine 4°C
Follow-up visit at 4–8 weeks after birth*	Infant, venous, or heel stick	2 ml heparinized, 6 capillary tubes (ideally), or at least one capillary tube	Parasitological examination	Max 6 hours at 4°C
	Siblings, finger prick	10 μl	Stat-Pak	-
		100 μl	ELISA	Filter paper 4°C
Follow-up visit at 10 months after birth*	Infant, venous or heel stick or digital puncture	3 ml EDTA, or at least one Eppendorf EDTA tube and one filter paper	Stat-Pak,	-
			T. Detect,
			ELISA	Filter paper 4°C, Plasma -20°C

*If at least one rapid test on cord blood is positive.