Figure 11.

Ultrastructural Analysis of Wortmannin Effects on BY-2 Cells.

(A) Golgi stack in an untreated cell. c, cis-cisterna; t, trans-cisterna.

(B) Size comparison of Golgi stacks and MVBs (arrows) in an untreated cell.

(C) High magnification of a multivesicular body in an untreated cell.

(D) and (E) Highly stainable plaques (arrowheads) at the cytoplasmic surface of MVBs in untreated cells.

(F) Golgi stack in a cell treated with wortmannin for 1 h. Stack parameters (number of cisternae, polarity) appear unchanged.

(G) and (H) MVBs (chevrons) swell and show reduced numbers of internal vesicles in wortmannin-treated cells.