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. 2004 Mar;16(3):672–693. doi: 10.1105/tpc.019703

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Copyright © 2004, American Society of Plant Biologists

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Figure 4. — Protein Gel Blot Analysis of Transgenic BY-2 Cells Expressing the YFP-BP-80 Reporter.

Lanes 3 to 6, anti-GFP detection of the full-length YFP-BP-80 reporter proteins in membrane (CM) fraction (lane 4, asterisk) and vacuole fraction (V) (lane 5, asterisk); lane 8, anti-BP-80 CT detection of the YFP-BP-80 reporter (asterisk) in vacuole fraction. The tentative degradation products derived from the reporter proteins are indicated by double asterisks (lanes 5 and 6). Wild-type proteins also were included as controls (lanes 1 and 2). CS, soluble fraction; P, pellet.