Figure 5.

Anti-VSR Colocalizes with the YFP-BP-80 Reporter but Is Separate from the GONST1-YFP Reporter.

Transgenic N. tabacum BY-2 cells expressing either YFP-BP-80 or GONST1-YFP reporters were fixed and incubated with either VSR, AtSRY21, or ManI antibodies to detect PVC and Golgi stacks, respectively. The primary antibodies were detected with rhodamine-conjugated secondary antibodies (red), whereas the two YFP reporters (green) were ready for detection in the confocal microscope using a 488-nm laser. When green and red images were superimposed, colocalization of two signals is indicated by a yellow color. Arrowheads and arrows indicate colocalization and separation, respectively. n, nucleus. Bar = 50 μm.