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BFA at 10 μg/mL Induces Structural Changes in YFP-Marked Golgi Stacks but Not in YFP-Marked PVCs.
Transgenic BY-2 cells expressing either the GONST1-YFP reporter (A) or the YFP-BP-80 reporter (B) were incubated with BFA at 10 μg/mL for up to 1 h. Treated cells then were collected at the times indicated for YFP imaging in the confocal laser scanning microscope. Arrows indicate BFA-induced aggregates in cells expressing the GONST1-YFP Golgi marker. n, nucleus. Bars = 50 μm.
