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Time Course of Wortmannin-Induced PVC Vacuole Formation.
(A) and (B) Transgenic BY-2 cell lines expressing the Golgi and PVC reporters were incubated with wortmannin at 33 μM for the times indicated. Samples of treated cells then were taken for YFP imaging. Arrowheads in (B) indicate wortmannin-induced vacuole formation. Insets are vacuolated YFP-BP-80–labeled PVCs. n, nucleus. Bars = 50 μm.
