Table 2.

Virucidal assay: effect of preincubation of N. latifolia extract with HSV-2

Incubation condition		Extracta	Virus titer (PFU)b
Temp (°C)	Duration (h)
37	0	-	5.56 × 105
37	0	+	3.52 × 105
37	2	-	5.29 × 104
37	2	+	3.42 × 104
4	2	-	4.88 × 105
4	2	+	6.03 × 105

^aConcentration: 33 μg/ml.

^bThe values are the means ± SD of data derived from three independent experiments performed in duplicate.