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Protein Phosphatase Activity in pp2aa2-1 and pp2aa3-1 Mutants
| MBP Phosphatase Activity (nmol/min/mg Protein)
|
||
|---|---|---|
| PP2A | PP1 | |
| Col | 294 ± 29 | 68 ± 7 |
| pp2aa2-1 | 341 ± 25 | 73 ± 14 |
| pp2aa3-1 | 318 ± 41 | 59 ± 17 |
Protein extracts from dark-grown seedlings were assayed for protein phosphatase activity with radioactively labeled myelin basic protein (MBP) as substrate (see Methods). Col, wild-type Columbia.
