Table 2.
Cotyledon Abnormalities in A Subunit Mutants
| Parental Genotype | Monocot | Cot Fusion | Tricot | Asymmetric | Other Abnorm. | Total Abnorm. | % Mutant (Observed)a | n (Total) |
|---|---|---|---|---|---|---|---|---|
| Ws | 0.4 | 0.4 | − | 472 | ||||
| rcn1 | 0.2 | 0.2 | 0.4 | 100 | 506 | |||
| Col | 0.4 | 0.4 | − | 498 | ||||
| pp2aa2-1 | <0.2 | 100 | 481 | |||||
| pp2aa3-1 | 0.2 | 0.2 | 100 | 485 | ||||
| rcn1 pp2aa2-1/+ | 0.7 | 3.7 | 0.5 | 1.5 | 6.3 | 25 (18) | 410 | |
| rcn1 pp2aa3-1/+ | 0.3 | 0.3 | 4.8 | 0.8 | 1.1 | 7.3 | 25 (23) | 357 |
| rcn1 pp2aa3-1 | 2.1 | 1.4 | 13.4 | 6.2 | 6.2 | 28.9 | 100 | 97 |
| pin1-6/+ | 4.0 | 4.5 | 4.3 | 0.3 | 13.1 | 25 | 398 | |
| rcn1 pin1-6/+ | 12.9 | 5.0 | 0.5 | 2.1 | 1.6 | 22.0 | 25 | 381 |
| pid-9/+ | 0.3 | 8.8 | 0.3 | 9.3 | 25 | 387 | ||
| rcn1 pid-9/+ | 0.7 | 0.7 | 13.7 | 0.4 | 0.4 | 16 | 25 | 278 |
Self-progeny of plants with the genotypes indicated were assayed for symmetry defects. For double mutant combinations that produce sterile plants, cotyledon phenotypes were scored in segregating families. Seedlings were grown for 7 d in the light on sterile medium and then scored for cotyledon abnormalities using a dissecting microscope. Numbers shown represent percentages (except those shown in italics). Blank spaces indicate that no seedlings exhibited the abnormal cotyledon phenotype specified. In all cases, a blank space indicates a value <0.26%.
In segregating families, 25% of the progeny are expected to be homozygous mutants. Numbers shown in parentheses indicate the observed percentage of progeny exhibiting the homozygous mutant phenotype.