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BHX causes cell apoptosis and G1 arrest. (A) A549 and MGC803 cells were treated with 5 μmol/L BHX for 72 h. Cell apoptosis was measured using Annexin V-FITC/PI staining. (B) A549 and MGC803 cells were treated with 5 μmol/L BHX for 24 h to 72 h. The percentage of cells in each cell cycle phase was determined by quantitation of DNA content by PI staining and flow cytometry. Data are presented as mean ± SD from three independent experiments.
