Figure 4.

Phenotypes Resulting from Crossing AvrL567 Transgenes into L. usitatissimum Lines with Different L Genes.

(A) A single T0 transgenic plant containing one copy of an AvrL567-A¹⁵⁰ transgene was crossed to L. usitatissimum lines homozygous for the L5, L6, L7, or Lx resistance gene as well as to the rust susceptible line Hoshangabad (Hosh). Twelve seeds from each cross as well as self-fertilized seeds were planted in soil. Photographs were taken 2 weeks after planting.

(B) Seeds resulting from a cross between a T0 transgenic plant containing one copy of the AvrL567-A¹²⁷ transgene and a L. usitatissimum line homozygous for L5. Approximately half of the seeds have a shrunken appearance (left) and are unable to germinate, whereas the phenotypically wild-type seeds (right) give rise to normal plants.

(C) Segregation of wild-type and dwarf phenotypes among 3-week-old seedlings resulting from a cross between a T0 transgenic plant containing one copy of the AvrL567-B¹²⁷ transgene and a L. usitatissimum line homozygous for L6.